Associated with international collaborative research work in “Host-Microbe Interaction using whole genome sequencing and Transcriptomics” at Corvallis, Oregon state University USA and participated.

2014-15 :

Awarded Sri Veerapaneni Narasimham Memorial Gold Medal for Best Research Scientist in the discipline of Plant Pathology for the year 2010 during XLIV Convocation of Acharya N.G.Ranga Agricultural University, Hyderabad on 20th March, 2014.

Established “Molecular Plant Pathology Lab” at Dept. of Plant Pathology, RARS, Tirupati and it was inaugurated by Dr. K. Raja Reddy, Director of Research, ANGRAU, Hyderabad on 09.01.2014. The Lab was equipped to carry work on Fungal Genomics.

Many external funded projects submitted to DBT, DST are under active consideration.

I have developed one mobile App “Greeshma” to cater the needs of Farming Community on crop Diseases which is very useful for Agricultural Officers, A.E.Os and Extension personnel. It was launched by Hon’ble Vice-Chancellor Dr.A.Padma Raju, ANGRAU, Hyderabad on 30th July, 2015.

I am involved in teaching at RARS, Tirupati taking course for Agriculture Polytechnic Students (DA-171).

I have completed one UGC major research project as Principal Investigator entitled “ Exploitation of Seed Endophytes for the Eco friendly Management of Aflatoxins in Maize- Aspergillus Pathosystem” from 2012-2015 with an outlay of 11,13,800/- was sanctioned on 25th July, 2012 for three years.

The results obtained under UGC Project, New Delhi was presented at International Plant Protection Congress, Berlin, Germany held during 24-27th August,2015, in which I was consulted by several International scientists to have collaborative research projects.

2015-16 :

Handle done UGC Major Research Project on Aflatoxin-Maize Pathosystem as Principal Investigator

Participated in National conference on Agri-Nanotechnology held during 20-22,March 2015 at RARS, Tirupati.

Participated in National Conference on“ National Priorities in Plant Health Management” held during February 4-5,2016 at RARS, Tirupati.

2016-17 :

Handled various UG, PG and Ph.D courses viz; PATH371(Diseases of Field crops),

PG courses viz; Mycology(PATH501) , Principles of Plant Pathology (PATH505)

Worked as NSS Programme Officer for the year 2016-17

2017-18 :

Acted as Head of KVK, Kalikiri and DAATTC, Kalikiri

Involved in extension activities of KVK, Kalikiri as Programme Co-Ordinator and Co-Ordinator, DAATTC, Kalikiri .

ExtensivelytouredinthecombineddistrictofChittoorunderdiagnosticfieldvisitstofarmers problem and addressed various pest and disease problems and their control measures.

Conductedseveraltrainingprogrammes,methoddemonstrations,groupdiscussionsonvarious aspects which have practical applicability and useful to the farming community.

2018-19 :

Acted as Head of KVK, Kalikiri and DAATTC, Kalikiri

Anadditionalareaof5acreswasbroughtundercultivationinadditiontotheexistinglandat KVK, Kalikiri.

Improved irrigation facilities at KVK by deepening off arm ponds and borewell

2019-20 :

Acted as Head of KVK, Kalikiri

Under digital Agriculture, developed one mobile app “Greeshma Plus” on various Field and Horticultural Crops and Livestock in a holistic way with an idea to provide information various aspects of Agriculture and Live stock to farming community.

2020-21 :

As an Administrator, continuous monitoring of construction work of Administrative building, Farmers hostel, Custom Hiring Centre and were inaugurated by Dr.Trilochan Mohapatra, Director General (ICAR) on 06.07.2020 at KVK, Kalikiri.

2021-22 :

Dealt Under graduate, post graduate and Ph.D courses like PATHAMBE101 (Agricultural Microbiology), PATH171 (Fundamentals of Plant Pathology) to UG students.

PG courses vix; Mycology, Principles of Plant Pathology were dealt

Ph.D courses viz; Advanced Mycology, Advanced Bacteriology, Advanced Virology, Host- Pathogen microbial interaction studies were dealt.

Chairman of Technical Sessions: Indian Phytopathological Society Central Zone Symposium and National Conference on Phytopathology for sustainable disease management and Food Security jointly conducted by IPS, New Delhi and ANGRAU held during 31st October- 2ndNovember,2022.

2022-23 :

Acted as External Examiner for Oral Comprehensive Exam of PG students at University of Agricultural Sciences, Banglore and College of Agriculture, Mandya during the year 2023.

Conducted workshop on“ Genetic Variability and Molecular characterization of plant pathogens as course Director in the Department of Plant Pathology, S.V. Agricultural College, Tirupatifrom28th October to 2nd November,2023

Organized National webinar on “Role of Nanotechnology in plant disease management” on 23rd August, 2023.

Worked as NSS Programme Officer-I for the year 2022-23

2023-24 :

International Collaborative Research Associated with International collaborative research work on Host-Microbe Interaction using whole genome sequencing and Transcriptomics at Corvallis, Oregon State University, USA from 01.03.2024 to 31.03.2024. He participated in International Conference on Soil borne Pathogens and American Phytopathological Society (APS) North Western Division Meeting and acted as judge for oral presentations held during March 26th - 28th, 2024 at Memorial Union Hall, Corvallis, Oregon State University.

Acted as Judge for Oral Presentations held during International Conference on Soil borne Plant Pathogens from 26th-28th March,2024 at Corvallis, Oregon State University, USA

Next generation sequencing and Transcriptomics, Host – microbe interaction studies.

⦁ Expertise in Next Generation Sequencing and Transcriptomics

⦁ ArtificialIntelligence_MachineLearninginComputationalBiology,BioinformaticsandBiology

⦁ Pythoncoding

⦁ International Collaborative Research Proposal on “Plant –Microbe Interaction Research Using Whole genome sequencing and Transcriptomics” in association with Oregon State University, USA.

⦁ Attended one month research programme at Oregon State University, Corvallis from 01.03.2024to 31.03.2024 as Visiting Scholar under J-1 Programme

Salient feature s of Research work :

ResistantandSusceptibilityofpotatogermplasmagainstPhytopthorainfestanswastested

Studies on mycological characters using Photomicrography system on P. infestans

MolecularcharacterizationofresistantandsusceptiblegermplasmagainstPVXvirususingmultiplex - PCR

Nanodrop spectrophotometer for DNA Quality assessment

Glass house Studies for germplasm evaluation against viral diseases

Whole Genomic Sequencing Studies & Transcriptomics was studied in Centre of Quantitative Life Sciences( CQLS)

CQLS Biocomputing and Bioinformatics facility provides wide-ranging resources, expertise and support for computational needs of the molecular biosciences community at Oregon State University.

The facility offers a robust computing environment for high-level computational biology and a versatile intellectual resource for interdisciplinary collaboration.

Services and Instrumentation

The CQLS Core Laboratory has services and instruments in the following areas:

Genomics– Shortread librarypreps , GBS library preparation and sequencing on the Illumina MiSeq and Next Seq 2000; Longread library preps for PacBioand services; Sanger sequencing and SSR fragment analysis on the 3730 DNA analyzer.

DNA/RNA extractions– Animal, plant, soil, King Fisher and custom extractions, High molecular weight DNA extractions

RNA/DNAQC and Analysis –QCandfragmentsizingonthe Agilent Bioanalyzer and Tape Station, and Blue Pippen size selection .

Microscopy– Keyence BZ-X710 fluorescent microscope, Leica Stellaris 5 Confocal Microscope System

Shared Instrumentation–BioTek Synergy H1Plate reader, Nano Drop One spectrophotometer, Qubit, ABI Absolute Qdigital PCR, ABI7500 real-time PCR, Azurec600GelImager, BioRupter, Speed Vac

Pacific Biosciences SequelII

Pac Bioofferstwogeneral types of sequencing,

Circular consensus sequencing (CCS)and

DNA is sheared for most CCS libraries to create libraries with ~10-20 kb fragment sizes that are short enough for the polymerase to pass over multiple times.

CLR sequencing, on the other hand, is optimized to create the longest reads possible. Since multiple passes over the same DNA molecule are not made due to the lengthofthe template, each read reflects one pass of the polymerase over the DNA molecule.

Depending on the size of the genomic DNA, shearing may not be necessary for this type of sequencing. Error rates are higher for this method.

CCS reads for whole genomes equencing projects are called HiFi reads.

Due to the narrow size selection required to target DNA fragment sizes suitable for CCS sequencing, a higher DNA input is required for HiFi libraries compared to standard or low input whole genome sequencing protocols.

A variety of library preparation options are available for Pac Biosequencing at the CQLS:

Whole genome sequencing

Low input options (with as little as 500 ng on input material) and standard options (5 ug of input material) are available. Low input options cannot be sequenced on more than one SMRT cell

Iso-Seqfor full-length RNA transcripts equencing

Multiplexed Microbial Libraries

Multiplex up to 16 microbes in one library with our standard pricing, or up to 48 microbes for an additional custom adapter fee.

Shotgun Metagenomic Libraries

Profile up to 4communitiesononeSMRTcell

BIOINFORMATICS

Annotation, orthe prediction of genes that encode proteins(CDS) and other genomic features such as

Command-Line Unix / Linux

Metagenomics

Phylogenetic Inference & Population Genetics

Sequence Analysis

Transcriptomics & RNA seq

BIOCOMPUTING

Advanced Cyber infrastructure Teaching Facility (ACTF)

On the Advanced Cyber infrastructure Teaching Facility (ACTF), learners and instructors explore computation at the big-data end of the spectrum, in topics ranging from Bioinformatics to Data Science.

Student Projects (utilizing the ACTF)

Delivered Lead talk on “Host –Microbe Interaction Studies using Next Generation Sequencing and Transcriptomics” at National Conference organised by Central Zone IPS, Rajendra Nagar, Hyderabad during 12-13th December, 2024.

Participated in the International Conference on Soil borne plant pathogens and APS Division meeting at Corvallis, Oregon State University, USA

Chairman of Technical Sessions: Annual combined Millet Group meeting conducted jointly IIMR, Hyderabad and ANGRAU held during 27th-28th June,2024 for Crop Protection section

Attended 4th National PG Conference as Subject Expert in Plant Pathology held at University of Agricultural Sciences, Raichur from 12th& 13th August,2024.

International/National Journals: 58

Conference proceedings:

Books and Book chapters:02

Technical bulletins: 08

Popular articles: 65

Any others:

Sri Veeravaneni Narasimham Memorial Gold Medal for Best Scienist in Plant Pathology, 2010